Second Hospital Affiliated to Lanzhou University

6 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Complete Thymectomy in Adult Rats with Non-invasive Endotracheal Intubation

Victoria R. Rendell¹, Charles Giamberardino², Jie Li², M. Louise Markert^2,3, Todd V. Brennan¹

¹Department of Surgery, Duke University Medical Center, ²Department of Pediatrics, Duke University Medical Center, ³Department of Immunology, Duke University Medical Center

Rodent thymectomy is a valuable technique in immunological research. Here, a protocol for complete thymectomy in adult rats using a mini-sternotomy along with non-invasive intubation and positive pressure ventilation to minimize perioperative morbidity and mortality is described.

Biology

Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs)

Johanna E. Kowalko¹, Li Ma², William R. Jeffery³

¹Genetics, Development and Cell Biology, Iowa State University, ²Department of Biological Sciences, University of Cincinnati, ³Department of Biology, University of Maryland

Gene-targeting mutagenesis is now possible in a wide range of organisms using genome editing techniques. Here, we demonstrate a protocol for targeted gene mutagenesis using transcription activator like effector nucleases (TALENs) in Astyanax mexicanus, a species of fish that includes surface fish and cavefish.

Neuroscience

Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult

Stephanie A. Herrlinger¹, Qiang Shao², Li Ma², Melinda Brindley³, Jian-Fu Chen²

¹Biomedical and Health Sciences Institute, University of Georgia, ²Center for Craniofacial Molecular Biology, University of Southern California, ³Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia

Here we describe a method for establishing a model of Zika virus-induced microcephaly in mouse. This protocol includes methods for embryonic, neonatal, and adult-stage intracerebral inoculation of the Zika virus.

Neuroscience

A Rat Model of Central Fatigue Using a Modified Multiple Platform Method

Weiyue Zhang^*1, Wei Zhang^*1, Ning Dai^*1, Chenxia Han², Fengzhi Wu¹, Xu Wang¹, Libo Tan¹, Jie Li¹, Feng Li¹, Qingjia Ren³

¹School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, ²Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, ³Institute of Tibetan Medicine, Tibetan Traditional Medical College

Here, we present a protocol to introduce a rat model of central fatigue using the modified multiple platform method (MMPM).

Developmental Biology

Incremental Temperature Changes for Maximal Breeding and Spawning in Astyanax mexicanus

Li Ma¹, Ruby Dessiatoun¹, Janet Shi¹, William R. Jeffery¹

¹Department of Biology, University of Maryland

This article outlines the basic laboratory conditions and protocols for an incremental temperature regime to stimulate maximal spawning in the Mexican tetra Astyanax mexicanus, which is an emerging model for developmental and evolutionary studies.

Medicine

Using Hyperbaric Oxygen to Improve the Radiosensitivity of Human U251 Glioma Cells

Li Ma^*1, Bin-qiang Ye^*1, Jie Li¹, Ya-ya Pei², Jia-wei Zhong², Fu-ling Mou³, Peng-fei Sun¹

¹Department of Radiotherapy, Second Hospital Affiliated to Lanzhou University, ²Department of Ultrasound, Second Hospital Affiliated to Lanzhou University, ³Department of Cardiology, Hubei Lichuan Dongfang Harmonious Hospital

This protocol shows that hyperbaric oxygen can enhance the proliferation inhibition and apoptosis of U251 glioma cells treated with X-ray irradiation by blocking the cells in the G2/M phase. This improves the radiosensitivity of human glioma cell lines.