Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology
2 ARTICLES PUBLISHED IN JoVE
Biology
Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
G. Grant Welstead 1, Tobias Brambrink 1, Rudolf Jaenisch 1
1Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology
This video shows the procedure for generating induced pluripotent stem cells using inducible lentivirus that express Oct4, Sox2, c-Myc and Klf4.
Immunology and Infection
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
Oktay Kirak 1, Eva-Maria Frickel 1, Gijsbert M. Grotenbreg 1,2, Heikyung Suh 1, Rudolf Jaenisch 1,3, Hidde L. Ploegh 1,3
1Whitehead Institute for Biomedical Research, 2Departments of Microbiology and Biological Sciences, National University of Singapore, 3Department of Biology, Massachusetts Institute of Technology
We demonstrate here that epigenetic reprogramming via Somatic Cell Nuclear Transfer (SCNT) can be used as a tool to generate mouse models with pre-defined T cell receptor (TCR) specificities. These transnuclear mice express the corresponding TCR from their endogenous locus under the control of the endogenous promoter.
