Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

2 ARTICLES PUBLISHED IN JoVE

Biology

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

G. Grant Welstead¹, Tobias Brambrink¹, Rudolf Jaenisch¹

¹Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

This video shows the procedure for generating induced pluripotent stem cells using inducible lentivirus that express Oct4, Sox2, c-Myc and Klf4.

Immunology and Infection

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Oktay Kirak¹, Eva-Maria Frickel¹, Gijsbert M. Grotenbreg^1,2, Heikyung Suh¹, Rudolf Jaenisch^1,3, Hidde L. Ploegh^1,3

¹Whitehead Institute for Biomedical Research, ²Departments of Microbiology and Biological Sciences, National University of Singapore, ³Department of Biology, Massachusetts Institute of Technology

We demonstrate here that epigenetic reprogramming via Somatic Cell Nuclear Transfer (SCNT) can be used as a tool to generate mouse models with pre-defined T cell receptor (TCR) specificities. These transnuclear mice express the corresponding TCR from their endogenous locus under the control of the endogenous promoter.