Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)Peter D. Burbelo 1, Kathryn H. Ching 1, Caitlin M. Klimavicz 1, Michael J. Iadarola 1
1Neurobiology and Pain Therapeutics Section, National Institute of Dental and Craniofacial Research, National Institutes of Health
The technical aspects of performing LIPS (Luciferase Immunoprecipitation Systems) are described. The overall approach involves expressing chimeric genes encoding antigens fused to Renilla luciferase (Ruc) in mammalian cells. Crude Ruc-antigen extracts are then prepared and, without purification, employed in immunoprecipitation assays to quantify antibodies.
