Studying Synaptic Vesicle Pools using Photoconversion of Styryl DyesFelipe Opazo 1, Silvio O. Rizzoli 1
1STED Microscopy of Synaptic Function, European Neuroscience Institute Göttingen
FM dyes have been of invaluable help in the understanding of synaptic dynamics. FMs are normally followed under the fluorescent microscope during different stimulation conditions. However, photoconversion of FM dyes combined with electron microscopy allows the visualization of distinct synaptic vesicle pools, among other ultrastructure components, in synaptic boutons.
