Institute for Medical Research Israel Canada, Hebrew University-Hadassah Medical School

3 ARTICLES PUBLISHED IN JoVE

Biology

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Oshri Avraham^*1, Sophie Zisman^*1, Yoav Hadas¹, Lilach Vald¹, Avihu Klar¹

¹Department of Medical Neurobiology, Institute for Medical Research Israel Canada, Hebrew University-Hadassah Medical School

This video demonstrates how to visualize axonal pathways of genetically defined groups of neurons in the embryonic chick spinal cord utilizing in ovo electroporation of reporter genes under the control of specific enhancer elements.

Neuroscience

Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

Ayelet Kohl¹, Yoav Hadas², Avihu Klar², Dalit Sela-Donenfeld¹

¹Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, ²Department of Medical Neurobiology, The Hebrew University of Jerusalem

How neuronal networks are established in the embryonic brain is a fundamental question in developmental neurobiology. Here we combined an electroporation technique with novel genetic tools, such as Cre/Lox–plasmids and PiggyBac-mediated DNA transposition system in the avian hindbrain to label dorsal interneurons and track their axonal projections and synaptic targets at various developmental stages.

Genetics

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

Keerat Kaur^1,2,3, Nishat Sultana^1,2,3, Yoav Hadas^1,2,3, Ajit Magadum^1,2,3, Mohammad Tofael Kabir Sharkar^1,2,3, Elena Chepurko^1,2,3, Lior Zangi^1,2,3

¹Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, ²Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai

This protocol presents a simple and coherent way to transiently upregulate a gene of interest using modRNA after myocardial infarction in mice.