University of Massachussetts

2 ARTICLES PUBLISHED IN JoVE

Biology

Karen A. Dunphy^1,2, Luwei Tao³, D. Joseph Jerry^1,2

¹Department of Veterinary and Animal Sciences, University of Massachussetts, ²Pioneer Valley Life Sciences institute, ³Molecular and Cellular Biology, University of Massachussetts

This article demonstrates the procedure developed by DeOme KB et al. (1959) and the sparing procedure developed by Brill B et al. (2008) for clearing the 4th inguinal mammary fat pad of a pubescent mouse in preparation for transplantation of mammary fragments, mammary epithelial cells, or mammary tumor cells.

Cancer Research

Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland

Dongxi Xiang^1,2, Luwei Tao^1,2, Zhe Li^1,2

¹Division of Genetics, Department of Medicine, Brigham and Women's Hospital, ²Department of Medicine, Harvard Medical School

The goal of this protocol is to describe a new breast cancer modeling approach based on the intraductal injection of Cre-expressing adenovirus into mouse mammary glands. This approach allows both cell-type- and organ-specific manipulation of oncogenic events in a temporally controlled manner.