NIH - National Institute of Health

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Non-invasive Imaging of Leukocyte Homing and Migration in vivo

Baomei Wang¹, Bernd H. Zinselmeyer^1,2, Jeremiah R. McDole¹, Peggy A. Gieselman¹, Mark J. Miller¹

¹Department of Pathology and Immunology, Washington University in St. Louis, ²National Institute of Neurological Disorders and Stroke, NINDS, NIH - National Institute of Health

Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.