College of Dentistry, New York University
2 ARTICLES PUBLISHED IN JoVE
Medicine
Technique to Collect Fungiform (Taste) Papillae from Human Tongue
Andrew I. Spielman *1, M. Yanina Pepino 2, Roy Feldman 3,4,5, Joseph G. Brand *4,6
1Department of Basic Science and Craniofacial Biology, College of Dentistry, New York University, 2Department of Internal Medicine and Department of Psychiatry, School of Medicine, Washington University in St. Louis, 3Veterans Affairs Medical Center, 4School of Dental Medicine, Department of Biochemistry, University of Pennsylvania-School of Medicine, 5Monell Chemical Senses Center, 6Monell Chemical Senses Center
Knowledge of molecular mechanisms underlying gustatory transduction has recently enjoyed significant advances, largely due to using animal models. However, the wide diversity in taste sensitivity and specificity among mammals warrants studies in human tissue. We describe a biopsy technique to collect living taste cells from the papillae on human tongue.
Neuroscience
Isolation and Culture of Human Fungiform Taste Papillae Cells
Hakan Ozdener 1, Andrew I. Spielman 2, Nancy E. Rawson 3
1Monell Chemical Senses Center, 2New York University College of Dentistry, 3AFB International
We aimed to develop a reproducible protocol for isolating and maintaining long-term cultures of human fungiform taste papillae cells. Cells from human fungiform papillae obtained by biopsy were successfully maintained in culture for more than eight passages (12 months) without loss of viability.
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