Changes in Mammary Gland Morphology and Breast Cancer Risk in RatsSonia de Assis 1, Anni Warri 1,2, M. Idalia Cruz 1, Leena Hilakivi-Clarke 1
1Department of Oncology, Georgetown University, 2Institute of Biomedicine, University of Turku Medical Faculty
Our protocol describes how to dissect the rat abdominal mammary gland and how to prepare mammary gland whole mounts. It also describes how to analyze mammary gland morphology using three end-points (number of terminal end buds, epithelial elongation and differentiation) and to use these results to predict mammary cancer risk in rats which were exposed to dietary modifications in utero or during prepuberty.
