The Walter and Eliza Hall Institute for Medical Research

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

Kirsty R. Short¹, Dimitri A. Diavatopoulos², Patrick C. Reading¹, Lorena E. Brown¹, Kelly L. Rogers³, Richard A. Strugnell¹, Odilia L.C. Wijburg¹

¹Department of Microbiology and Immunology, University of Melbourne, ²Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, ³The Centre for Dynamic Imaging, The Walter and Eliza Hall Institute for Medical Research

A concurrent infection with influenza A virus is one of the factors implicated in the induction of invasive pneumococcal disease during asymptomatic Streptococcus pneumoniae carriage. Here we describe a mixed infection method using infant mice to investigate the synergism between these two respiratory pathogens.

Bioengineering

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

Joseph Dragavon¹, Carolyn Sinow², Alexandra D. Holland¹, Abdessalem Rekiki¹, Ioanna Theodorou³, Chelsea Samson⁴, Samantha Blazquez¹, Kelly L. Rogers⁵, Régis Tournebize^1,6,7, Spencer L. Shorte¹

¹Plate-Forme d'Imagerie Dynamique, Imagopole, Institut Pasteur, ²Department of Radiation Oncology, Stanford School of Medicine, ³Service Hospitalier Frédéric Joliot, Institut d'Imagerie Biomédicale, ⁴Vanderbilt School of Medicine, ⁵The Walter & Eliza Hall Institute of Medical Research, ⁶Unité INSERM U786, Institut Pasteur, ⁷Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur

Expanding the foundation and applicability of Fluorescence by Unbound Excitation from Luminescence (FUEL) by surveying the relevant principles and demonstrating its compatibility with a multitude of fluorophores and antibody-targeted conditions.

Medicine

Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice

Matthias Ernst^1,2,3, Adele Preaudet¹, Tracy Putoczki^1,2

¹The Walter and Eliza Hall Institute for Medical Research, ²The Department of Medical Biology, University of Melbourne, ³Olivia Newton-John Cancer Research Institute

We describe methods for longitudinal monitoring of the efficacy of therapeutics for the treatment of colonic pathologies in mice using a rigid endoscope. This protocol can be readily used for the characterization of the therapeutic response of an individual tumor in live mice and also for monitoring potential disease relapse.