University of Miami, Miller School of Medicine
3 ARTICLES PUBLISHED IN JoVE
Neuroscience
Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration
Yousuf O. Ali 1, Wilfredo Escala 1, Kai Ruan 1, R. Grace Zhai 1
1Department of Molecular and Cellular Pharmacology, University of Miami, Miller School of Medicine
Behavior assays for measuring locomotor functions, learning, and memory abilities in Drosophila.
Biology
Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
Nathalie Baumlin-Schmid 1, Matthias Salathe 1, Nevis L. Fregien 2
1Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, University of Miami, Miller School of Medicine, 2Department of Cell Biology, University of Miami, Miller School of Medicine
Primary human bronchial epithelial cells are difficult to transduce. This protocol describes the production of lentiviruses and their concentration as well as the optimal culture conditions necessary to achieve highly efficient transductions in these cells throughout differentiation to a pseudostratified epithelium.
Neuroscience
Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ
Jennifer M. Brazill 1, Yi Zhu 1, Chong Li 1, R. Grace Zhai 1,2
1Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, 2School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University
We have developed a simple and adaptable workflow to extract quantitative data from fluorescence-imaging-based cell biological studies of protein aggregation and autophagic flux in the central nervous system of Drosophila models of neurodegeneration.
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