Georgia Health Sciences University

2 ARTICLES PUBLISHED IN JoVE

Biology

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

Erhard Bieberich¹

¹Institute of Molecular Medicine and Genetics, Georgia Health Sciences University

To test the interaction of a protein with its target lipid we used MACS and Annexin V-conjugated magnetic beads and lipid vesicles synthesized from the target lipid and Annexin V-binding phosphatidylserine. Proteins bound to the target lipid are co-purified and analyzed after elution from the beads.

Neuroscience

Creating Objects and Object Categories for Studying Perception and Perceptual Learning

Karin Hauffen^1,2,3, Eugene Bart⁴, Mark Brady⁵, Daniel Kersten⁶, Jay Hegdé^1,2,3

¹Brain and Behavior Discovery Institute, Georgia Health Sciences University, ²Vision Discovery Institute, Georgia Health Sciences University, ³Department of Opthalmology, Georgia Health Sciences University, ⁴Intelligent Systems Laboratory, Palo Alto Research Center, ⁵Pattern Recognition Systems, Palo Alto Research Center, ⁶Department of Psychology, University of Minnesota

We describe a novel methodology for creating naturalistic 3-D objects and object categories with precisely defined feature variations. We use simulations of the biological processes of morphogenesis and phylogenesis to create novel, naturalistic virtual 3-D objects and object categories that can then be rendered as visual images or haptic objects.