National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Sang Beom Jun^1,2, Verginia Cuzon Carlson¹, Stephen Ikeda³, David Lovinger¹

¹Section on Synaptic Pharmacology/Laboratory for Integrative Neuroscience, National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism, ²Department of Electronics Engineering, Ewha Womans University, ³Section on Transmitter Signaling/Laboratory of Molecular Physiology, National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism

This report demonstrates a technique for mechanical isolation of individual viable neurons retaining attached presynaptic boutons. Vibrodissociated neurons have the advantages of rapid production, excellent pharmacological control and improved space-clamp without influence from neighboring cells. This method can be used for imaging of synaptic elements and patch-clamp recording.

Neuroscience

Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

Youjin Lee^*1,2, Daeho Ryu^*3, Saeyeong Jeon³, Yena Lee¹, Yoon Kyung Cho¹, Chang-Hyeon Ji^1,2, Yong-Kweon Kim^3,4, Sang Beom Jun^1,2,5

¹Department of Electronic and Electrical Engineering, Ewha Womans University, ²Graduate Program in Smart Factory, Ewha Womans University, ³Department of Electrical and Computer Engineering, Seoul National University, ⁴Graduate School of Engineering Practice, Seoul National University, ⁵Department of Brain and Cognitive Sciences, Ewha Womans University

Here, we present the fabrication method of an optrode system with optical fibers for light delivery and an electrode array for neural recording. In vivo experiments with transgenic mice expressing channelrhodopsin-2 show the feasibility of the system for simultaneous optogenetic stimulation and electrophysiological recording.