In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial CellsJason Letourneau 1, Cynthia Levesque 1, Frederic Berthiaume 1, Mario Jacques 1, Michael Mourez 1
1Universite de Montreal, Groupe de Recherche sur les Maladies Infectieuses du Porc GREMIP, Faculte de medecine veterinaire
This protocol is a simple bacterial adhesion assay consisting in counting the numbers of bacterial colony forming units that are adhered onto cultured cells. The assay is robust, independent of the adhesin studied, and numerous variations are used in most laboratories working on bacterial pathogenesis.
