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Sigma-Aldrich

5 ARTICLES PUBLISHED IN JoVE

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Biology

Optimized PCR-based Detection of Mycoplasma
Paige L. Dobrovolny 1, Dan Bess 1
1Product Management, Sigma-Aldrich

The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma, Acholeplasma, and Ureaplasma contamination in cell cultures and other cell culture derived biologicals.

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Biology

FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability
Deborah A. Blake 1, Nicolai V. Bovin 2, Dan Bess 1, Stephen M. Henry 1
1Biotechnology Research Institute, AUT University and KODE Biotech Ltd, 2Shemyakin Institute of Bioorganic Chemistry RAS, Moscow, Russia

Function-Spacer-Lipid (FSL) constructs allow the surface characteristics of living cells and virions to be modified without loss of vitality. The method requires only simple contact of an FSL construct solution with a cell/virion and spontaneous and stable surface incorporation occurs.

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Biology

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Matthew J. Coussens *1, Kevin Forbes *1, Carol Kreader *1, Jack Sago 1, Carrie Cupp 1, John Swarthout 1
1Sigma-Aldrich

The Target ID Library is a plasmid-based, genome-wide collection of cloned cDNA used to identify miRNA targets. Here we demonstrate its use and application.

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Biology

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
Keith Hansen 1, Matthew J. Coussens 1, Jack Sago 1, Shilpi Subramanian 1, Monika Gjoka 1, Dave Briner 1
1Emerging Technologies, Sigma Life Science

The CompoZr Custom Zinc-Finger Nuclease (ZFN) Service enables precise genome editing in any organism or cell line at any locus defined by the user. This article describes the process for the design, manufacture, validation and implementation of the CompoZr Custom ZFN Service.

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Biology

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
Matthew J. Coussens 1, Courtney Corman 1, Ashley L. Fischer 1, Jack Sago 1, John Swarthout 1
1Sigma-Aldrich

Here we use a human LentiPlex pooled library and traditional sequencing methods to identify gene targets promoting cell survival. We demonstrate how to set up and deconvolute a LentiPlex screen and validate the results.

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