<p class="jove_content">A novel and highly efficient two-step affinity chromatography protocol has been developed and is described in detail. The method is based on a small purification tag with two inherent affinities and is applicable to a wide range of target proteins with different properties.

	 
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orthogonal-protein-purification-facilitated-small-bispecific-affinity

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Due to the high costs associated with purification of recombinant proteins the protocols need to be rationalized. For high-throughput efforts there is a ...

Royal Institute of Technology

1 ARTICLES PUBLISHED IN JoVE

Biology