Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human PapillomavirusXuelian Wang 1, William W. Greenfield 2, Hannah N. Coleman 3, Lindsey E. James 3, Mayumi Nakagawa 3
1Department of Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University , 2Department of Obstetrics and Gynecology, College of Medicine, University of Arkansas for Medical Sciences , 3Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences
Characterizing T-cell epitopes of pathogens that cause localized infections such as human papillomavirus is a challenge because of limited number of T cells in circulation. A method is described in which rare T cells were isolated and were characterized starting with a very small number of cells.
