Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation AssayDerek Tilley 1, Irina Levit 2, John A. Samis 1,3
1Applied Bioscience Program, Faculty of Science, University of Ontario Institute of Technology , 2Nursing Program, Faculty of Health Sciences, University of Ontario Institute of Technology , 3Medical Laboratory Science Program, Faculty of Health Sciences, University of Ontario Institute of Technology
This study describes a novel microplate assay that measures FV coagulation activity during fibrin clot formation in human plasma which has not been reported previously. The method uses a kinetic microplate reader to continuously measure the change in absorbance at 405nm during fibrin clot formation in human plasma.
