University of Liverpool
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
Sebastian Kirchner 1, Joanne L Fothergill 2, Elli A. Wright 1, Chloe E. James 1, Eilidh Mowat 1, Craig Winstanley 1
1Institute of Infection and Global Health, University of Liverpool , 2NIHR Biomedical Research Centre in Microbial Disease, University of Liverpool
Current diagnostic antimicrobial susceptibility testing relies on the planktonic growth of isolates in nutrient rich, aerobic conditions. Here, we employ an alternative artificial sputum medium to study antimicrobial susceptibility of Pseudomonas aeruginosa biofilms under both aerobic and microaerophilic conditions more representative of the cystic fibrosis lung.
Medicine
Experimental Human Pneumococcal Carriage
Jenna F. Gritzfeld 1, Angie D. Wright 1,2,3, Andrea M. Collins 1,2,4, Shaun H. Pennington 1, Adam K.A. Wright 5, Aras Kadioglu 6, Daniela M. Ferreira 1, Stephen B. Gordon 1
1Respiratory Infection Group, Liverpool School of Tropical Medicine, 2Royal Liverpool and Broadgreen, University Hospital Trust, 3Comprehensive Local Research Network, 4NIHR Biomedical Research Centre in Microbial Diseases, Royal Liverpool and Broadgreen University Hospitals NHS Trust, 5Institute of Lung Health, Respiratory Biomedical Unit, University Hospitals of Leicester NHS Trust & University of Leicester, 6Department of Clinical Infection Microbiology & Immunology, Institute of Infection & Global Health, University of Liverpool
Experimental human pneumococcal carriage offers a natural model of carriage and a potential model for use in vaccine development. This technique is valuable yet complex and involves clinical risk by introducing a pathogen into a human. We have developed a detailed protocol.
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