KU Leuven, Belgium

2 ARTICLES PUBLISHED IN JoVE

Medicine

The Use of Cystometry in Small Rodents: A Study of Bladder Chemosensation

Pieter Uvin^1,2,3, Wouter Everaerts^1,2,3, Silvia Pinto^2,3, Yeranddy A. Alpízar^2,3, Mathieu Boudes^1,2,3, Thomas Gevaert^1,2,3, Thomas Voets^2,3, Bernd Nilius^2,3, Karel Talavera^2,3, Dirk De Ridder^1,3

¹Laboratory of Experimental Urology, Department of Development and Regeneration, KU Leuven, Belgium, ²Laboratory for Ion Channel Research, Department of Cellular and Molecular Medicine, KU Leuven, Belgium, ³TRP Research Platform Leuven (TRPLe), KU Leuven, Belgium

Cystometry is an efficient technique to measure bladder function of small animals in vivo. The bladder is continuously infused at rates controlled through an intravesical catheter, whereas the urethra is left free for micturition. This allows for repetitive filling and emptying of the bladder, while intravesical pressure and voided volume are recorded.

Immunology and Infection

Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging

Noémie Luyts¹, Greetje Vande Velde², Matthias Vanneste¹, Helene De Bruyn¹, Annelies Janssens¹, Natalie Verstraeten³, Thomas Voets¹, Wouter Everaerts⁴

¹Laboratory of Ion Channel Research (LICR), VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium & Department of Cellular and Molecular Medicine, KU Leuven, ²Biomedical MRI, Department of Imaging & Pathology, KU Leuven, ³Belgium & KU Leuven Centre of Microbial and Plant Genetics, VIB-KU Leuven Center for Microbiology, Leuven, ⁴Laboratory of Experimental Urology, Department of Development and Regeneration, KU Leuven

This manuscript describes the intravesical administration of uropathogenic bacteria with a lux operon to induce a urinary tract infection in mice and subsequent longitudinal in vivo analysis of the bacterial load using bioluminescence imaging.