Albert Einstein College of Medicine, Yeshiva University
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Revealing Neural Circuit Topography in Multi-Color
Stacey L. Reeber 1, Samrawit A. Gebre 1, Nika Filatova 1, Roy V. Sillitoe 1
1Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University
We provide a practical guide for delivering tracers in vivo and use the spinocerebellar pathway as a model system to demonstrate essential steps for successful neuronal circuit analysis in mice. We describe in detail our versatile tracing protocol that exploits wheat germ agglutinin (WGA) conjugated to Alexa fluorophores.
Neuroscience
Wholemount Immunohistochemistry for Revealing Complex Brain Topography
Joshua J. White 1, Stacey L. Reeber 1, Richard Hawkes 2, Roy V. Sillitoe 1
1Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University , 2Department of Cell Biology and Anatomy and the Hotchkiss Brain Institute, Faculty of Medicine, University of Calgary
Neural circuits are topographically organized into functional compartments with specific molecular profiles. Here, we provide the practical and technical steps for revealing global brain topography using a versatile wholemount immunohistochemical staining approach. We demonstrate the utility of the method using the well-understood cytoarchitecture and circuitry of cerebellum.
