University of Toledo
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Automated Quantification of Synaptic Fluorescence in C. elegans
Brianne L. Sturt 1, Bruce A. Bamber 1
1Department of Biological Sciences, University of Toledo
The abundance of neurotransmitter receptors clustered at synapses strongly influences synaptic strength. This method quantifies fluorescently-labeled neurotransmitter receptors in three dimensions with single-synapse resolution in C. elegans, allowing hundreds of synapses to be rapidly characterized within a single sample without distortions introduced by z-plane projection.
Neuroscience
Experimental Methods for Testing the Effects of Neurotrophic Peptide, ADNF-9, Against Alcohol-induced Apoptosis during Pregnancy in C57BL/6 Mice
Youssef Sari 1
1Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo
The experimental designs proposed here focus on studying the effects of alcohol exposure in apoptosis and the application of neurotrophic peptide during pregnancy in fetal brain. A detailed description from the breeding to the collection of fetal brains is described. Techniques for determination of apoptosis are also described in detail.
