Royal Liverpool and Broadgreen University Hospital Trust
2 ARTICLES PUBLISHED IN JoVE
Medicine
Bronchoalveolar Lavage (BAL) for Research; Obtaining Adequate Sample Yield
Andrea M. Collins 1,2, Jamie Rylance 3, Daniel G. Wootton 4, Angela D. Wright 3,5, Adam K. A. Wright 1,3, Duncan G. Fullerton 3,6, Stephen B. Gordon 3
1Biomedical Research Centre in Microbial Diseases, National Institute for Health Research, 2Respiratory Infection Group, Royal Liverpool and Broadgreen University Hospital Trust, 3Respiratory Infection Group, Liverpool School of Tropical Medicine, 4Institute of Infection and Global Health, University of Liverpool, 5Comprehensive Local Research Network, Royal Liverpool and Broadgreen University Hospital Trust, 6Department of Respiratory Research, University Hospital Aintree
We describe a research technique for fiberoptic bronchoscopy and bronchoalveolar lavage using low pressure suction. The technique is used to harvest immune cells from the lung bronchoalveolar surfaces. Local anesthetic and mild conscious sedation (midazolam) is used. Subjects tolerate the procedure well and experience minimal side effects.
Medicine
Experimental Human Pneumococcal Carriage
Jenna F. Gritzfeld 1, Angie D. Wright 1,2,3, Andrea M. Collins 1,2,4, Shaun H. Pennington 1, Adam K.A. Wright 5, Aras Kadioglu 6, Daniela M. Ferreira 1, Stephen B. Gordon 1
1Respiratory Infection Group, Liverpool School of Tropical Medicine, 2Royal Liverpool and Broadgreen, University Hospital Trust, 3Comprehensive Local Research Network, 4NIHR Biomedical Research Centre in Microbial Diseases, Royal Liverpool and Broadgreen University Hospitals NHS Trust, 5Institute of Lung Health, Respiratory Biomedical Unit, University Hospitals of Leicester NHS Trust & University of Leicester, 6Department of Clinical Infection Microbiology & Immunology, Institute of Infection & Global Health, University of Liverpool
Experimental human pneumococcal carriage offers a natural model of carriage and a potential model for use in vaccine development. This technique is valuable yet complex and involves clinical risk by introducing a pathogen into a human. We have developed a detailed protocol.
