St Jude Children's Research Hospital

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain

Ida Annunziata^*1, Annette Patterson^*1, Alessandra d'Azzo¹

¹Department of Genetics, St Jude Children's Research Hospital

This procedure illustrates how to isolate from the adult mouse brain the mitochondria-associated ER membranes or MAMs and the glycosphingolipid-enriched microdomain fractions from MAMs and mitochondrial preparations.