Pyrosequencing for Microbial Identification and CharacterizationPatrick J. Cummings 1, Ray Ahmed 2, Jeffrey A. Durocher 2, Adam Jessen 2, Tamar Vardi 2, Kristina M. Obom 1
1Center for Biotechnology Education, Krieger School of Arts and Sciences, Johns Hopkins University, 2Qiagen Sciences, Inc.
Pyrosequencing is a versatile technique that facilitates microbial genome sequencing that can be used to identify bacterial species, discriminate bacterial strains, and detect genetic mutations that confer resistance to anti-microbial agents. In this video, the procedure for microbial amplicon generation, amplicon pyrosequencing, and DNA sequence analysis will be demonstrated.
