Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
Caghan Kizil 1, Anne Iltzsche 1, Jan Kaslin 1, Michael Brand 1
1DFG-Center for Regenerative Therapies Dresden, Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden
In this article, we demonstrate a method for manipulation of gene expression in the ventricular cells of the adult zebrafish telencephalon using antisense morpholino oligonucleotides. We present this method as an efficient and quick protocol that can be used for functional studies in the adult vertebrate brain.
Neuroscience
Modeling Amyloid-β42 Toxicity and Neurodegeneration in Adult Zebrafish Brain
Prabesh Bhattarai 1, Alvin Kuriakose Thomas 2, Mehmet Ilyas Cosacak 1, Christos Papadimitriou 1, Violeta Mashkaryan 3, Yixin Zhang 2, Caghan Kizil 1,3
1German Centre for Neurodegenerative Diseases (DZNE) Dresden within Helmholtz Association, 2B CUBE, Center for Molecular Bioengineering, Technische Universität Dresden, 3Center for Regenerative Therapies Dresden (CRTD), TU Dresden
This protocol describes the synthesis, characterization, and injection of monomeric amyloid-β42 peptides for generating amyloid toxicity in adult zebrafish to establish an Alzheimer's disease model, followed by histological analyses and detection of aggregations.
