Universidade Federal do Rio Grande do Sul

2 ARTICLES PUBLISHED IN JoVE

Behavior

Simultaneous EEG Monitoring During Transcranial Direct Current Stimulation

Pedro Schestatsky^1,2,3, Leon Morales-Quezada^3,4, Felipe Fregni³

¹Programa de Pós-Graduação em Ciências Médica, Universidade Federal do Rio Grande do Sul, ²Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), ³Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital and Massachusetts General Hospital, Harvard Medical School, ⁴De Montfort University

Transcranial direct current stimulation (tDCS) is a non-invasive brain stimulation technique that has shown initial therapeutic effects in several neurological conditions. The main mechanism underlying these therapeutic effects is the modulation of cortical excitability. Therefore, online monitoring of cortical excitability would help guide stimulation parameters and optimize its therapeutic effects. In the present article we review the use of a novel device that combines simultaneous tDCS and EEG monitoring in real time.

Biochemistry

Measurement of Glutamate Uptake using Radiolabeled L-[³H]-Glutamate in Acute Transverse Slices Obtained from Rodent Resected Hippocampus

Débora G. Souza¹, Yasmine Nonose¹, Diogo O. Souza^1,2, Roberto F. Almeida^3,4

¹Graduate Program in Biochemistry, Universidade Federal do Rio Grande do Sul, ²Department of Biochemistry, Universidade Federal do Rio Grande do Sul, ³Department of Biological Sciences, Universidade Federal de Ouro Preto, ⁴Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal de Ouro Preto

This article describes a reliable and simple way to obtain ex vivo acute hippocampal transverse slices from mice and rats using a tissue chopper. Slices obtained from resected hippocampi can be submitted for functional glutamate uptake analysis to investigate glutamatergic system homeostasis.