Zentrum für operative Medizin der Universität Würzburg
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model
Malgorzata Burek 1, Ellaine Salvador 1, Carola Y. Förster 1
1Klinik und Poliklinik für Anästhesiologie, University of Wurzburg
This method describes how to isolate and immortalize microvascular endothelial cells from mouse brain. We describe a step-by-step protocol starting from the homogenization of brain tissue, digestion steps, seeding and immortalization of the cells. Usually, it takes about five weeks to obtain a homogenous, immortalized microvascular endothelial cell line.
Medicine
Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier
Ellaine Salvador 1, Winfried Neuhaus 1,2, Carola Foerster 1
1Klinik und Poliklinik für Anästhesiologie, Zentrum für operative Medizin der Universität Würzburg, 2Department of Medicinal Chemistry, Faculty of Life Sciences, University of Vienna
In vitro traumatic brain injury models are being developed to reproduce in vivo brain deformation. Stretch-induced injury has been employed for astrocytes, neurons, glial cells, aortic, and brain endothelial cells. However, our system uses a blood brain barrier (BBB) model that possesses properties constituting a legitimate model of the BBB to establish an in vitro TBI model.
