Hertie Institute for Clinical Brain Research
2 ARTICLES PUBLISHED IN JoVE
The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease
Lena F. Burbulla 1,2, Rejko Krüger 1,2
1DZNE, German Center for Neurodegenerative Diseases, 2Department of Neurodegenerative Diseases, Laboratory of Functional Neurogenomics, Hertie Institute for Clinical Brain Research
Fibroblasts from patients carrying mutations in Parkinson's disease-causing genes represent an easily accessible ex vivo model to study disease-associated phenotypes. Live cell imaging gives the opportunity to study morphological and functional parameters in living cells. Here we describe the preparation of human fibroblasts and subsequent monitoring of mitochondrial phenotypes .
Immunology and Infection
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
Christina Stoeckle 1,2, Ioanna A. Rota 1, Eva Tolosa 3, Christoph Haller 4, Arthur Melms 5, Eleni Adamopoulou 1
1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen
This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
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