CEA DSV iRCM SCSR

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

Olivier Etienne^1,2,3,4, Amandine Bery^1,2,3,4, Telma Roque^1,2,3,4, Chantal Desmaze^1,2,3,4, François D. Boussin^1,2,3,4

¹Laboratoire de Radiopathologie, CEA DSV iRCM SCSR, ²INSERM, U967, ³Université Paris Diderot, Sorbonne Paris Cité, ⁴Université Paris Sud, UMR 967

Administration of two analogs of thymidine, EdU and BrdU, in pregnant mice allows the analysis of cell cycle progression in neural and progenitor cells in the embryonic mouse brain. This method is useful to determine the effects of genotoxic stress, including ionizing radiation, during brain development.

Neuroscience

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Mathieu Daynac^*1,2,3,4,5, Lise Morizur^*1,2,3,4, Thierry Kortulewski^1,2,3,4, Laurent R. Gauthier^1,2,3,4, Martial Ruat⁵, Marc-André Mouthon^1,2,3,4, François D. Boussin^1,2,3,4

¹CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, UMR 967, ²INSERM, UMR 967, ³Université Paris Diderot, Sorbonne Paris Cité, UMR 967, ⁴Université Paris Sud, UMR 967, ⁵CNRS, Université Paris Sud, UMR 9197, Neuroscience Paris-Saclay Institute, Molecules Circuits Department

We report a Fluorescent Activated Cell Sorting (FACS)-based method to isolate neural stem cells (NSCs) and their progeny from the subventricular zone (SVZ) of the adult mouse brain. Applied to Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) transgenic mice, it allows the study of cell cycle progression by live imaging.