An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegansBruno B. Queliconi 1, Alicia J. Kowaltowski 1, Keith Nehrke 2
1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, 2Department of Medicine, Nephrology Division, University of Rochester Medical Center, School of Medicine and Dentistry
A protocol is described that uses anoxia/starvation in C. elegans to model ischemia/reperfusion. Functional outcomes include increased mortality, visible abnormalities in GFP-labeled neuronal processes, and impaired behavioral responses that require neuronal function.
