IRCCS Fondazione Santa Lucia
2 ARTICLES PUBLISHED IN JoVE
Biology
Generation of Myospheres From hESCs by Epigenetic Reprogramming
Sonia Albini 1, Pier Lorenzo Puri 1,2
1Muscle Development and Regeneration Program, Sanford-Burnham Institute for Medical Research, 2IRCCS Fondazione Santa Lucia
Here, we describe a protocol based on epigenetic reprogramming of human embryonic stem cells (hESCs) toward generating a homogeneous population of skeletal muscle progenitors that under permissive culture conditions form three-dimensional clusters of contractile myofibers (myospheres), which recapitulate biological features of human skeletal muscles.
Biology
In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
Francesco Castagnetti 1,2, Elisabetta Fiacco 3, Carol Imbriano 4, Lucia Latella 1,2
1Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences, University of Modena and Reggio Emilia
Active autophagy is associated with productive muscle regeneration, which is essential for Muscle Stem Cell (MuSC) activation. Here, we provide a protocol for the in situ detection of LC3, an autophagy marker in MyoD-positive MuSCs of muscle tissue sections from control and injured mice.
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