INSERM U872
2 ARTICLES PUBLISHED IN JoVE
Biology
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
Miquella G. Chavez 1,2, Jimmy Hu 1, Kerstin Seidel 1, Chunying Li 1,3, Andrew Jheon 1, Adrien Naveau 4,5,6, Orapin Horst 1,7, Ophir D. Klein 1,8
1Department of Orofacial Sciences and Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, 2Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 3Department of Pathology and Research Center, Zhongshan Hospital of Dalian University, 4Université Paris Descartes, Sorbonne Paris Cite, UMR S872, 5Centre de Recherche des Cordeliers, Université Pierre et Marie Curie, UMR S872, 6INSERM U872, 7Division of Endodontics, Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, 8Department of Pediatrics and Institute for Human Genetics, University of California, San Francisco
The continuously growing mouse incisor provides a model for studying renewal of dental tissues from dental epithelial stem cells (DESCs). A robust system for consistently and reliably obtaining these cells from the incisor and expanding them in vitro is reported here.
Medicine
Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models
Jeremie Cosette 1,2,3, Rym Ben Abdelwahed 2, Sabrina Donnou-Triffault 1,4,5, Catherine Sautès-Fridman 2, Patrice Flaud *3, Sylvain Fisson *1,4,5
1Genethon, 2Cordeliers Research Center, INSERM U872, 3Matter and Complex Systems Laboratory, Université Paris Diderot, 4INSERM U951, 5UMR_S951, Université d'Evry Val d'Essonne
Bioluminescence imaging is a well-known tool for localizing tumors and metastases, but quantification of these images often requires complex calculations and particular instruments. We describe the easy-to-use luminoscore method, based on precise acquisition conditions, requiring no calculations, and enabling tumor burden and treatment response to be monitored in mouse models.
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