Commissariat à l'énergie atomique et aux énergies alternatives (CEA) Saclay, France

1 ARTICLES PUBLISHED IN JoVE

Biology

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Natalie J. Saez¹, Hervé Nozach², Marilyne Blemont¹, Renaud Vincentelli¹

¹Architecture et Fonction des Macromolécules Biologiques (AFMB), Aix-Marseille Université, ²iBiTec-S, Service d'Ingénierie Moléculaire des Protéines (SIMOPRO), Commissariat à l'énergie atomique et aux énergies alternatives (CEA) Saclay, France

A protocol for the quantitative, high throughput expression screening and analytical purification of fusion proteins from small-scale Escherichia coli cultures is described and applied to the expression of disulfide-rich animal venom protein targets.