Erasmus MC - University Medical Center

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

Tahsin Stefan Barakat¹, Joost Gribnau¹

¹Department of Reproduction and Development, Erasmus MC - University Medical Center

Fluorescent in situ hybridization (FISH) allows the detection of nucleic acids in their native environment within cells. We here describe a protocol for the combined, simultaneous detection of RNA and DNA by means of FISH, which can be used to study X chromosome inactivation in mouse embryonic stem cells.