Clemson University Eukaryotic Pathogens Innovation Center

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Sarah Bauer¹, Meghan Conlon¹, Meredith Morris¹

¹Department of Genetics and Biochemistry, Clemson University Eukaryotic Pathogens Innovation Center

Glycosome dynamics in African trypanosomes are difficult to study by traditional cell biology techniques such as electron and fluorescence microscopy. As a means of observing dynamic organelle behavior, a fluorescent-organelle reporter system has been used in conjunction with flow cytometry to monitor real-time glycosome dynamics in live parasites.