Charité Universitätmedizin
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons
Sam A. Booker 1, Jie Song 1, Imre Vida 1
1Institute of Integrative Neuroanatomy, NeuroCure Cluster of Excellence, Charité Universitätmedizin
Cortical networks are controlled by a small, but diverse set of inhibitory interneurons. Functional investigation of interneurons therefore requires targeted recording and rigorous identification. Described here is a combined approach involving whole-cell recordings from single or synaptically-coupled pairs of neurons with intracellular labeling, post-hoc morphological and immunocytochemical analysis.
Neuroscience
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
Paul Turko 1, Keenan Groberman 1, Toralf Kaiser 2, Yuchio Yanagawa 3, Imre Vida 1
1Institut für Integrative Neuroanatomie, Charité, Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center, Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University
This protocol describes a cell sorting based method for the purification and culture of fluorescent GABAergic or glutamatergic neurons from the neocortex and hippocampus of postnatal mice or rats.
