Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Jeffrey Kroon^*1, Anna E. Daniel^*1, Mark Hoogenboezem¹, Jaap D. van Buul¹

¹Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam

Leukocytes cross the endothelial monolayer using the paracellular or the transcellular route. We developed a simple assay to follow the distribution of endogenous junctional VE-cadherin and PECAM-1 during leukocyte transendothelial migration under physiological flow to discriminate between the two transmigration routes.