German Center for Cardiovascular Research (DZHK) partner site Goettingen
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
Eva Wagner *1,2,3, Sören Brandenburg *1,2, Tobias Kohl 1,2, Stephan E. Lehnart 1,2,3,4
1Heart Research Center Goettingen, 2Clinic of Cardiology & Pulmonology, University Medical Center Goettingen, 3German Center for Cardiovascular Research (DZHK) partner site Goettingen, 4BioMET, Center for Biomedical Engineering & Technology, University of Maryland School of Medicine
In cardiac myocytes, tubular membrane structures form intracellular networks. We describe optimized protocols for i) isolation of myocytes from mouse heart including quality control, ii) live cell staining for state-of-the-art fluorescence microscopy, and iii) direct image analysis to quantify the component complexity and the plasticity of intracellular membrane networks.
Biochemistry
Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
Andreas Andric 1, Eva Wagner 1, Anne Heberle 1, Max Holzknecht 1, Alexander K. H. Weiss 1
1Research Institute for Biomedical Aging Research, University of Innsbruck
This protocol describes how to extract fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) from swine kidney and mouse liver. The listed methods may be adapted to other proteins of interest and modified for other tissues.
