CNRS UMR 7241, INSERM U1050, Collège de France

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

Ulrike Pannasch^*1, Jérémie Sibille^*1,2, Nathalie Rouach¹

¹Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050, Collège de France, ²Paris Diderot University

The preparation of acute brain slices from isolated hippocampi, as well as the simultaneous electrophysiological recordings of astrocytes and neurons in stratum radiatum during stimulation of schaffer collaterals is described. The pharmacological isolation of astroglial potassium and glutamate transporter currents is demonstrated.

Medicine

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue

Elena Dossi¹, Thomas Blauwblomme^2,3, Rima Nabbout^2,4, Gilles Huberfeld^2,5, Nathalie Rouach¹

¹Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050, Collège de France, ²Infantile Epilepsies & Brain Plasticity, INSERM U1129, PRES, Paris Descartes University, Sorbonne Paris Cité, CEA, ³Neurosurgery Department, Necker Hospital, AP-HP, Paris Descartes University, ⁴Rare Epilepsies Reference Center, Necker Hospital, AP-HP, Paris Descartes University, ⁵Neurophysiology Department, La Pitié-Salpêtrière Hospital, AP-HP, Sorbonne and Pierre and Marie Curie University

We here describe how to perform multi-electrode array recordings of human epileptic cortical tissue. Epileptic tissue resection, slice preparation and multi-electrode array recordings of interictal and ictal events are demonstrated in detail.