USDA-ARS Dairy Forage Research Center

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Glass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens

Hana T. Millen¹, Jordan C. Gonnering¹, Ryan K. Berg², Susan K. Spencer³, William E. Jokela³, John M. Pearce⁴, Jackson S. Borchardt¹, Mark A. Borchardt³

¹Wisconsin Water Science Center, United States Geological Survey, ²University of Wisconsin – Madison, ³Agricultural Research Service, United States Department of Agriculture, ⁴Alaska Science Center, United States Geological Survey

Glass wool filters have been used to concentrate waterborne viruses by a number of research groups around the world. Here we show a simple approach for constructing glass wool filters and demonstrate the filters are also effective in concentrating waterborne viral, bacterial and protozoan pathogens.

JoVE Core

Measurement of Greenhouse Gas Flux from Agricultural Soils Using Static Chambers

Sarah M. Collier¹, Matthew D. Ruark², Lawrence G. Oates^3,4, William E. Jokela⁵, Curtis J. Dell⁶

¹Office of Sustainability, University of Wisconsin-Madison, ²Department of Soil Science, University of Wisconsin-Madison, ³Department of Agronomy, University of Wisconsin-Madison, ⁴Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, ⁵USDA-ARS Dairy Forage Research Center, ⁶USDA-ARS Pasture Systems Watershed Management Research Unit

This article showcases the static chamber-based method for measurement of greenhouse gas flux from soil systems. With relatively modest infrastructure investments, measurements may be obtained from multiple treatments/locations and over timeframes ranging from hours to years.