Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

Mahdieh Tabatabaei Shafiei¹, Catalina M. Carvajal Gonczi¹, Mohammed Samiur Rahman², Ashley East³, Jonathan François³, Peter J. Darlington^1,3

¹Department of Biology, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, ²Department of Chemistry and Biochemistry, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, ³Department of Exercise Science, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University

Periodic acid Schiff staining is a technique that visualizes the polysaccharide content of tissues. This article demonstrates periodic acid Schiff staining protocol adapted for use on peripheral blood mononuclear cells purified from human venous blood. Such samples are enriched for lymphocytes and other white blood cells of the immune system.