San Raffaele Scientific Institute and Vita-Salute University

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Federica Daniele¹, Eliana S. Di Cairano¹, Stefania Moretti¹, Giovanni Piccoli², Carla Perego^1,3

¹Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, ²San Raffaele Scientific Institute and Vita-Salute University, ³CEND Center of Excellence in Neurodegenerative Diseases, Università degli Studi di Milano

This paper provides a method for investigating neurotransmitter vesicle dynamics in neuroblastoma cells, using a synaptobrevin2-pHluorin construct and Total Internal Reflection Fluorescence Microscopy. The strategy developed for image processing and data analysis is also reported.