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University of Ontario Institute of Technology

2 ARTICLES PUBLISHED IN JoVE

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Education

Metal-free Synthesis of Ynones from Acyl Chlorides and Potassium Alkynyltrifluoroborate Salts
Cassandra L. Taylor 1, Yuri Bolshan 1
1Faculty of Science, University of Ontario Institute of Technology

The goal of this manuscript is to demonstrate a straightforward method for the preparation of ynones from acyl chloride and potassium alkynyltrifluoroborate salt starting materials. The one-pot reaction proceeds rapidly in the presence of boron trichloride without exclusion of air and moisture.

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Biochemistry

Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
Richard V. Augimeri 1, Andrew J. Varley 1, Janice L. Strap 1
1Molecular Microbial Biochemistry Laboratory, Faculty of Science (Applied Bioscience Program), University of Ontario Institute of Technology

The protocols outlined herein facilitate the convenient investigation of bacterial ethylene responses by utilizing 2-chloroethylphosphonic acid (CEPA). Ethylene is produced in situ through the decomposition of CEPA in an aqueous bacterial growth medium, circumventing the requirement for pure ethylene gas.

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