University of Wisconsin–Madison
2 ARTICLES PUBLISHED IN JoVE
Medicine
Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
Christopher J. Johnson 1, Christina M. Carlson 2, Aaron R. Morawski 3, Alyson Manthei 4, Neil R. Cashman 5
1USGS National Wildlife Health Center, 2Department of Soil Science, University of Wisconsin–Madison, 3Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4Merial Veterinary Scholars Program, School of Veterinary Medicine, University of Wisconsin–Madison, 5Department of Neurology, University of British Columbia
Measuring the barrier to the interspecies transmission of prion diseases is challenging and typically involves animal challenges or biochemical assays. Here, we present an in vitro prion protein conversion assay with the ability to predict species barriers.
Biology
Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)
Graham S. Erwin 1, Matthew P. Grieshop 1, Devesh Bhimsaria 1,2, Asuka Eguchi 3, José A. Rodríguez-Martínez 1, Aseem Z. Ansari 1,4
1Department of Biochemistry, University of Wisconsin–Madison, 2Department of Electrical and Computer Engineering, University of Wisconsin–Madison, 3Graduate Program in Cellular and Molecular Biology, University of Wisconsin–Madison, 4The Genome Center, University of Wisconsin–Madison
Identifying the direct targets of genome-targeting molecules remains a major challenge. To understand how DNA-binding molecules engage the genome, we developed a method that relies on crosslinking of small molecules to isolate chromatin (COSMIC).
