VHHSC
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay
Simon Wang 1, SooHyun Yoo 1, Hae-yoon Kim 1, Mannan Wang 2, Clare Zheng 2, Wade Parkhouse 2, Charles Krieger 2, Nicholas Harden 1
1Department of Molecular Biology and Biochemistry, Simon Fraser University, 2Department of Biomedical Physiology and Kinesiology, Simon Fraser University
This protocol demonstrates how Proximity Ligation Assay can be used to detect in situ protein-protein interactions at the Drosophila larval neuromuscular junction. With this technique, Discs large and Hu-li tai shao are shown to form a complex at the postsynaptic region, an association previously identified through co-immunoprecipitation.
Medicine
Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice
Kyle Peake 1, John Manning 1, Coral-Ann Lewis 1,2, Christine Barr 1, Fabio Rossi 2, Charles Krieger 1,3
1Department of Biomedical Physiology and Kinesiology, Simon Fraser University, 2The Biomedical Research Centre, University of British Columbia, 3Division of Neurology, Department of Medicine, Neuromuscular Disease Unit, VHHSC
We describe a protocol whereby busulfan conditioning permits the bone marrow of a recipient mouse to be replaced with bone marrow cells from donor mice ubiquitously expressing green fluorescent protein, in the absence of irradiation. This technique is useful to study bone marrow cell accumulation in the central nervous system.
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