CHUV, Université de Montréal, St-Hyacinthe, QC
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings
Patrick Burns 1, Hai Lun Liu 2, Shikha Kuthiala 2, Gilles Fecteau 1, André Desrochers 1, Lucien Daniel Durosier 2, Mingju Cao 2, Martin G. Frasch 2,3,4
1Département de sciences cliniques, CHUV, Université de Montréal, St-Hyacinthe, QC, 2Département d'obstetriques et de gynécologie, CHU Ste-Justine Research Centre, Université de Montréal, 3Département de neurosciences, CHU Ste-Justine Centre de recherche, Université de Montréal, 4Centre de recherche en reproduction animale (CRRA), Université de Montréal, St-Hyacinthe, QC
The chronically instrumented non-anesthetized fetal sheep model is used to study human fetal development in health and disease, because it permits surgical placement and maintenance of catheters and electrodes, repetitive blood sampling, substance injection, recording of bioelectrical activity, and in vivo imaging. We describe the procedures required to establish this model.
Medicine
A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence
Eric Parent 1, Patrick Burns 2, André Desrochers 1, Martine Boulianne 1
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, 2University of Bristol
Here we present a protocol to surgically create 'intestinal ligated loops' in chicken small intestines. This procedure allows for the comparison of multiple Clostridium perfringens strains' virulence in situ in a single host. This method markedly decreases the number of chickens usually necessary for similar in vivo experiments.
