Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B CellsGisela Nogales-Gadea *1,2, Abhishek Saxena *1, Carolin Hoffmann *1, Judith Hounjet 1, Daniëlle Coenen 1, Peter Molenaar 1, Mario Losen 1, Pilar Martinez-Martinez 1
1School for Mental Health and Neuroscience, Maastricht University, 2Department of Neurosciences, Institut d'Investigació Germans Trias i Pujol
Synthesis of human monoclonal antibodies is the first step in studies aimed at unraveling the pathophysiological mechanisms of auto-antibody-mediated immune responses. We have developed a protocol to generate recombinant human immunoglobulin G (IgG) monoclonal antibodies from blood sorted B cells, including B-cell isolation, antibody cloning and in vitro synthesis.
