Leidos Biomedical Research, Inc.
2 ARTICLES PUBLISHED IN JoVE
Biology
SpOT the Correct Tissue Every Time in Multi-tissue Blocks
Anna Coffey 1,2, Michael D. Johnson 3, Deborah L. Berry 3
1Center for Advanced Preclinical Research (CAPR), Frederick National Laboratory for Cancer Research, 2Leidos Biomedical Research, Inc., 3Department of Oncology, Georgetown University
The purpose of the Specimen Orientation Tag (SpOT) is to function as an orientation tool to aid in individual tissue identification in multi-tissue paraffin blocks. These protocols demonstrate how it is constructed easily from common, low-cost histology materials and serves as a reliable visual marker in paraffin blocks and sections.
Cancer Research
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
Robert Y. S. Cheng 1, Nimit L. Patel 2, Timothy Back 1, Debashree Basudhar 1, Veena Somasundaram 1, Joseph D. Kalen 2, David A. Wink 1, Lisa A. Ridnour 1
1Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute at Frederick, 2Small Animal Imaging Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc.
This work presets an advanced protocol to accurately assess tumor loading by detection of green fluorescent protein and bioluminescence signals as well as the integration of quantitative molecular detection technique.
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