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University of Science & Technology Beijing

2 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold
Lindsay N. Nail 1, Dawei Zhang 2,3, Jessica L. Reinhard 1, Melissa A. Grunlan 1,2
1Department of Biomedical Engineering, Texas A&M University, 2Department of Material Science and Engineering, Texas A&M University, 3Institute of Advanced Materials and Technology, University of Science & Technology Beijing

Scaffolds capable of fitting within cranio-maxillofacial (CMF) bone defects while exhibiting osteoconductivity and bioactivity are of interest. This protocol describes the preparation of a shape memory scaffold based on polycaprolactone diacrylate (PCL-DA) using a solvent-casting particulate-leaching (SCPL) method employing a fused salt template and application of a bioactive polydopamine coating.

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Biochemistry

Real-time Tracking of DNA Fragment Separation by Smartphone
Chunxian Tao 1, Bo Yang 1, Zhenqing Li 1, Dawei Zhang 1, Yoshinori Yamaguchi 1,2,3
1Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, 2Department of Applied Physics, Graduate School of Engineering, Osaka University, 3East China University of Science and Technology, Department of Physics Faculty of Science

Traditional slab gel electrophoresis (SGE) experiments require a complicated apparatus and high chemical consumption. This work presents a protocol that describes a low-cost method to separate DNA fragments within a short timeframe.

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